Volatile codes: Correlation of olfactory signals and reception in Drosophila-yeast chemical communication.

Drosophila have evolved strong mutualistic associations with yeast communities that best support their growth and survival, resulting in the development of novel niches. It has been suggested that flies recognize their cognate yeasts primarily based on the rich repertoire of volatile organic compounds (VOCs) derived from the yeasts. Thus, it remained an exciting avenue to study whether fly spp. detect and discriminate yeast strains based on odor alone, and if so, how such resolution is achieved by the olfactory system in flies. We used two fly species known to exploit different niches and harboring different yeasts, D. suzukii (a pest of fresh fruit) and D. melanogaster (a saprophytic fly and a neurogenetic model organism). We initially established the behavioral preference of both fly species to six Drosophila-associated yeasts; then chemically analyzed the VOC profile of each yeast which revealed quantitative and qualitative differences; and finally isolated and identified the physiologically active constituents from yeast VOCs for each drosophilid that potentially define attraction. By employing chemical, behavioral, and electrophysiological analyses, we provide a comprehensive portrait of the olfactory neuroethological correlates underlying fly-yeast coadaptation in two drosophilids with distinct habitats

Volatile codes: Correlation of olfactory signals and reception in Drosophila-yeast chemical communication

O estudo de caso retrata a atuação profissional de egressos, modalidade presencial, do Curso de Administração (2011-2014), da Universidade Federal de Santa Catarina (CAD-UFSC). Da população de 548 bacharéis deste Survey, obteve-se a amostra de 146 investigados, num Intervalo de Confiança de 95% e Erro Amostral de 7% (RENDER, STAIR JR.; HANNA, 2010). O questionário foi estruturado com perguntas fechadas Estilo Likert (HAIR JR., ANDERSON; TATHAM; BLACK, 2005), fixado no Google Docs e enviado convite por e-mail aos egressos do período em questão. A coleta de dados foi do mês de maio a junho de 2015. O tratamento foi quantitativo com auxílio do SPSS®. A pesquisa apontou que predominam os egressos na faixa etária de 26 a 30 anos (50,7%), sendo 50,7% do sexo feminino, a maioria natural do Estado de Santa Catarina, cursaram o ensino médio em escola privada e que cerca de 30% fez intercâmbio no exterior durante a graduação. Eles trabalham em empresas de médio porte e a faixa salarial oscila de 3 a 6 salários mínimos. A maioria fez ou cursa pós-graduação. Eles realçaram a relevância de graduarem-se na UFSC pelo reconhecimento pessoal e profissional, reforçando as avaliações do CAD-UFSC em 5 Estrela pelo MEC

Volatile codes: Correlation of olfactory signals and reception in Drosophila-yeast chemical communication.

Drosophila have evolved strong mutualistic associations with yeast communities that best support their growth and survival, resulting in the development of novel niches. It has been suggested that flies recognize their cognate yeasts primarily based on the rich repertoire of volatile organic compounds (VOCs) derived from the yeasts. Thus, it remained an exciting avenue to study whether fly spp. detect and discriminate yeast strains based on odor alone, and if so, how such resolution is achieved by the olfactory system in flies. We used two fly species known to exploit different niches and harboring different yeasts, D. suzukii (a pest of fresh fruit) and D. melanogaster (a saprophytic fly and a neurogenetic model organism). We initially established the behavioral preference of both fly species to six Drosophila-associated yeasts; then chemically analyzed the VOC profile of each yeast which revealed quantitative and qualitative differences; and finally isolated and identified the physiologically active constituents from yeast VOCs for each drosophilid that potentially define attraction. By employing chemical, behavioral, and electrophysiological analyses, we provide a comprehensive portrait of the olfactory neuroethological correlates underlying fly-yeast coadaptation in two drosophilids with distinct habitats

Survey of Aedes triseriatus (Diptera: Culicidae) for Lacrosse Encephalitis Virus and West Nile Virus in Lorain County, Ohio

From June through September 2003, we conducted a survey of female Aedes triseriatus (Say) for infection with La Crosse encephalitis virus (family Bunyaviridae, genus Orthobunyavirus, LACV) and West Nile virus (family Flaviviridae, genus Flavivirus, WNV) at three locations in Lorain County, Ohio. To determine infection rate and seasonal variation of both viruses in the Ae. triseriatus population, Ae. triseriatus were collected weekly by using gravid traps and CO2-baited CDC light traps and tested for virus by using reverse transcriptase polymerase chain reaction. In total, 170 pools comprised of 2,143 females were tested for LACV, of which seven were positive; the maximum likelihood estimate of infection rate combined throughout the season was 3.22/1,000. None of 170 pools comprised of 2,158 females tested for WNV were positive. LACV-positive pools were detected between late July and early September